The Cerebellin 4 Precursor Gene Is a Direct Target of SRY and SOX9 in Mice
Identifieur interne : 008043 ( Main/Exploration ); précédent : 008042; suivant : 008044The Cerebellin 4 Precursor Gene Is a Direct Target of SRY and SOX9 in Mice
Auteurs : Stephen T. Bradford [Australie] ; Ryuji Hiramatsu [Japon] ; Madhavi P. Maddugoda [Australie] ; Pascal Bernard [Australie] ; Marie-Christine Chaboissier [France] ; Andrew Sinclair [Australie] ; Andreas Schedl [France] ; Vincent Harley [Australie] ; Yoshiakira Kanai [Japon] ; Peter Koopman [Australie] ; Dagmar Wilhelm [Australie]Source :
- Biology of reproduction [ 0006-3363 ] ; 2009.
Descripteurs français
- Pascal (Inist)
English descriptors
- KwdEn :
Abstract
In most mammals, the expression of SRY (sex-determining region on the Y chromosome) initiates the development of testes, and thus determines the sex of the individual. However, despite the pivotal role of SRY, its mechanism of action remains elusive. One important missing piece of the puzzle is the identification of genes regulated by SRY. In this study we used chromatin immunoprecipitation to identify direct SRY target genes. Anti-mouse SRY antibody precipitated a region 7.5 kb upstream of the transcriptional start site of cerebellin 4 precursor (Cbln4), which encodes a secreted protein. Cbln4 is expressed in Sertoli cells in the developing gonad, with a profile mimicking that of the testis-determining gene SRY-box containing gene 9 (Sox9). In transgenic XY mouse embryos with reduced Sox9 expression, Cbln4 expression also was reduced, whereas overexpression of Sox9 in XX mice caused an upregulation of Cbln4 expression. Finally, ectopic upregulation of SRY in vivo resulted in ectopic expression of Cbln4. Our findings suggest that both SRY and SOX9 contribute to the male-specific upregulation of Cbln4 in the developing testis, and they identified a direct in vivo target gene of SRY.
Url:
Affiliations:
- Australie, France, Japon
- Provence-Alpes-Côte d'Azur, Région de Kantō, Victoria (État)
- Melbourne, Nice, Tokyo
- Université de Melbourne, Université de Tokyo
Links toward previous steps (curation, corpus...)
- to stream PascalFrancis, to step Corpus: 002940
- to stream PascalFrancis, to step Curation: 003643
- to stream PascalFrancis, to step Checkpoint: 002788
- to stream Main, to step Merge: 008757
- to stream Pmc, to step Corpus: 001780
- to stream Pmc, to step Curation: 001640
- to stream Pmc, to step Checkpoint: 002715
- to stream Ncbi, to step Merge: 000529
- to stream Ncbi, to step Curation: 000529
- to stream Ncbi, to step Checkpoint: 000529
- to stream Main, to step Merge: 008205
- to stream Main, to step Curation: 008043
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en" level="a">The Cerebellin 4 Precursor Gene Is a Direct Target of SRY and SOX9 in Mice</title>
<author><name sortKey="Bradford, Stephen T" sort="Bradford, Stephen T" uniqKey="Bradford S" first="Stephen T." last="Bradford">Stephen T. Bradford</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hiramatsu, Ryuji" sort="Hiramatsu, Ryuji" uniqKey="Hiramatsu R" first="Ryuji" last="Hiramatsu">Ryuji Hiramatsu</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Veterinary Anatomy, The University of Tokyo</s1>
<s2>Tokyo</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
<settlement type="city">Tokyo</settlement>
</placeName>
<orgName type="university">Université de Tokyo</orgName>
</affiliation>
</author>
<author><name sortKey="Maddugoda, Madhavi P" sort="Maddugoda, Madhavi P" uniqKey="Maddugoda M" first="Madhavi P." last="Maddugoda">Madhavi P. Maddugoda</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bernard, Pascal" sort="Bernard, Pascal" uniqKey="Bernard P" first="Pascal" last="Bernard">Pascal Bernard</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Human Molecular Genetics Laboratory, Prince Henry's Institute of Medical Research, Monash Medical Centre</s1>
<s2>Clayton, Victoria</s2>
<s3>AUS</s3>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Clayton, Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chaboissier, Marie Christine" sort="Chaboissier, Marie Christine" uniqKey="Chaboissier M" first="Marie-Christine" last="Chaboissier">Marie-Christine Chaboissier</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Institut National de la Santé et de la Recherche Médicale, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="05"><s1>U636, and Laboratoire de Genetique du Developpement Normal et Pathologique, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sinclair, Andrew" sort="Sinclair, Andrew" uniqKey="Sinclair A" first="Andrew" last="Sinclair">Andrew Sinclair</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Paediatrics, Murdoch Children's Research Institute, University of Melbourne, Royal Children's Hospital</s1>
<s2>Melbourne, Victoria</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Melbourne, Victoria</wicri:noRegion>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schedl, Andreas" sort="Schedl, Andreas" uniqKey="Schedl A" first="Andreas" last="Schedl">Andreas Schedl</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Institut National de la Santé et de la Recherche Médicale, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="05"><s1>U636, and Laboratoire de Genetique du Developpement Normal et Pathologique, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Harley, Vincent" sort="Harley, Vincent" uniqKey="Harley V" first="Vincent" last="Harley">Vincent Harley</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Human Molecular Genetics Laboratory, Prince Henry's Institute of Medical Research, Monash Medical Centre</s1>
<s2>Clayton, Victoria</s2>
<s3>AUS</s3>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Clayton, Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kanai, Yoshiakira" sort="Kanai, Yoshiakira" uniqKey="Kanai Y" first="Yoshiakira" last="Kanai">Yoshiakira Kanai</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Veterinary Anatomy, The University of Tokyo</s1>
<s2>Tokyo</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
<settlement type="city">Tokyo</settlement>
</placeName>
<orgName type="university">Université de Tokyo</orgName>
</affiliation>
</author>
<author><name sortKey="Koopman, Peter" sort="Koopman, Peter" uniqKey="Koopman P" first="Peter" last="Koopman">Peter Koopman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wilhelm, Dagmar" sort="Wilhelm, Dagmar" uniqKey="Wilhelm D" first="Dagmar" last="Wilhelm">Dagmar Wilhelm</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">INIST</idno>
<idno type="inist">10-0036251</idno>
<date when="2009">2009</date>
<idno type="stanalyst">PASCAL 10-0036251 INIST</idno>
<idno type="RBID">Pascal:10-0036251</idno>
<idno type="wicri:Area/PascalFrancis/Corpus">002940</idno>
<idno type="wicri:Area/PascalFrancis/Curation">003643</idno>
<idno type="wicri:Area/PascalFrancis/Checkpoint">002788</idno>
<idno type="wicri:explorRef" wicri:stream="PascalFrancis" wicri:step="Checkpoint">002788</idno>
<idno type="wicri:doubleKey">0006-3363:2009:Bradford S:the:cerebellin:precursor</idno>
<idno type="wicri:Area/Main/Merge">008757</idno>
<idno type="wicri:source">PMC</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2804802</idno>
<idno type="RBID">PMC:2804802</idno>
<idno type="wicri:Area/Pmc/Corpus">001780</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001780</idno>
<idno type="wicri:Area/Pmc/Curation">001640</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001640</idno>
<idno type="wicri:Area/Pmc/Checkpoint">002715</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">002715</idno>
<idno type="wicri:Area/Ncbi/Merge">000529</idno>
<idno type="wicri:Area/Ncbi/Curation">000529</idno>
<idno type="wicri:Area/Ncbi/Checkpoint">000529</idno>
<idno type="wicri:doubleKey">0006-3363:2009:Bradford S:the:cerebellin:precursor</idno>
<idno type="wicri:Area/Main/Merge">008205</idno>
<idno type="wicri:Area/Main/Curation">008043</idno>
<idno type="wicri:Area/Main/Exploration">008043</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a">The Cerebellin 4 Precursor Gene Is a Direct Target of SRY and SOX9 in Mice</title>
<author><name sortKey="Bradford, Stephen T" sort="Bradford, Stephen T" uniqKey="Bradford S" first="Stephen T." last="Bradford">Stephen T. Bradford</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hiramatsu, Ryuji" sort="Hiramatsu, Ryuji" uniqKey="Hiramatsu R" first="Ryuji" last="Hiramatsu">Ryuji Hiramatsu</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Veterinary Anatomy, The University of Tokyo</s1>
<s2>Tokyo</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
<settlement type="city">Tokyo</settlement>
</placeName>
<orgName type="university">Université de Tokyo</orgName>
</affiliation>
</author>
<author><name sortKey="Maddugoda, Madhavi P" sort="Maddugoda, Madhavi P" uniqKey="Maddugoda M" first="Madhavi P." last="Maddugoda">Madhavi P. Maddugoda</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Bernard, Pascal" sort="Bernard, Pascal" uniqKey="Bernard P" first="Pascal" last="Bernard">Pascal Bernard</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Human Molecular Genetics Laboratory, Prince Henry's Institute of Medical Research, Monash Medical Centre</s1>
<s2>Clayton, Victoria</s2>
<s3>AUS</s3>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Clayton, Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Chaboissier, Marie Christine" sort="Chaboissier, Marie Christine" uniqKey="Chaboissier M" first="Marie-Christine" last="Chaboissier">Marie-Christine Chaboissier</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Institut National de la Santé et de la Recherche Médicale, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="05"><s1>U636, and Laboratoire de Genetique du Developpement Normal et Pathologique, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sinclair, Andrew" sort="Sinclair, Andrew" uniqKey="Sinclair A" first="Andrew" last="Sinclair">Andrew Sinclair</name>
<affiliation wicri:level="4"><inist:fA14 i1="06"><s1>Department of Paediatrics, Murdoch Children's Research Institute, University of Melbourne, Royal Children's Hospital</s1>
<s2>Melbourne, Victoria</s2>
<s3>AUS</s3>
<sZ>6 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Melbourne, Victoria</wicri:noRegion>
<orgName type="university">Université de Melbourne</orgName>
<placeName><settlement type="city">Melbourne</settlement>
<region type="état">Victoria (État)</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Schedl, Andreas" sort="Schedl, Andreas" uniqKey="Schedl A" first="Andreas" last="Schedl">Andreas Schedl</name>
<affiliation wicri:level="3"><inist:fA14 i1="04"><s1>Institut National de la Santé et de la Recherche Médicale, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="3"><inist:fA14 i1="05"><s1>U636, and Laboratoire de Genetique du Developpement Normal et Pathologique, Université de Nice-Sophia Antipolis</s1>
<s2>Nice</s2>
<s3>FRA</s3>
<sZ>5 aut.</sZ>
<sZ>7 aut.</sZ>
</inist:fA14>
<country>France</country>
<placeName><region type="region">Provence-Alpes-Côte d'Azur</region>
<region type="old region">Provence-Alpes-Côte d'Azur</region>
<settlement type="city">Nice</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Harley, Vincent" sort="Harley, Vincent" uniqKey="Harley V" first="Vincent" last="Harley">Vincent Harley</name>
<affiliation wicri:level="1"><inist:fA14 i1="03"><s1>Human Molecular Genetics Laboratory, Prince Henry's Institute of Medical Research, Monash Medical Centre</s1>
<s2>Clayton, Victoria</s2>
<s3>AUS</s3>
<sZ>4 aut.</sZ>
<sZ>8 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Clayton, Victoria</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Kanai, Yoshiakira" sort="Kanai, Yoshiakira" uniqKey="Kanai Y" first="Yoshiakira" last="Kanai">Yoshiakira Kanai</name>
<affiliation wicri:level="4"><inist:fA14 i1="02"><s1>Department of Veterinary Anatomy, The University of Tokyo</s1>
<s2>Tokyo</s2>
<s3>JPN</s3>
<sZ>2 aut.</sZ>
<sZ>9 aut.</sZ>
</inist:fA14>
<country>Japon</country>
<placeName><settlement type="city">Tokyo</settlement>
<region type="région">Région de Kantō</region>
<settlement type="city">Tokyo</settlement>
</placeName>
<orgName type="university">Université de Tokyo</orgName>
</affiliation>
</author>
<author><name sortKey="Koopman, Peter" sort="Koopman, Peter" uniqKey="Koopman P" first="Peter" last="Koopman">Peter Koopman</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wilhelm, Dagmar" sort="Wilhelm, Dagmar" uniqKey="Wilhelm D" first="Dagmar" last="Wilhelm">Dagmar Wilhelm</name>
<affiliation wicri:level="1"><inist:fA14 i1="01"><s1>Institute for Molecular Bioscience, The University of Queensland</s1>
<s2>Brisbane, Queensland</s2>
<s3>AUS</s3>
<sZ>1 aut.</sZ>
<sZ>3 aut.</sZ>
<sZ>10 aut.</sZ>
<sZ>11 aut.</sZ>
</inist:fA14>
<country>Australie</country>
<wicri:noRegion>Brisbane, Queensland</wicri:noRegion>
</affiliation>
</author>
</analytic>
<series><title level="j" type="main">Biology of reproduction</title>
<title level="j" type="abbreviated">Biol. reprod.</title>
<idno type="ISSN">0006-3363</idno>
<imprint><date when="2009">2009</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
<seriesStmt><title level="j" type="main">Biology of reproduction</title>
<title level="j" type="abbreviated">Biol. reprod.</title>
<idno type="ISSN">0006-3363</idno>
</seriesStmt>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animal</term>
<term>Development</term>
<term>Gene</term>
<term>Mouse</term>
<term>Reproduction</term>
<term>Sertoli cell</term>
<term>Sex determination</term>
<term>Testicle</term>
</keywords>
<keywords scheme="Pascal" xml:lang="fr"><term>Gène</term>
<term>Développement</term>
<term>Cellule Sertoli</term>
<term>Déterminisme sexe</term>
<term>Testicule</term>
<term>Reproduction</term>
<term>Animal</term>
<term>Souris</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">In most mammals, the expression of SRY (sex-determining region on the Y chromosome) initiates the development of testes, and thus determines the sex of the individual. However, despite the pivotal role of SRY, its mechanism of action remains elusive. One important missing piece of the puzzle is the identification of genes regulated by SRY. In this study we used chromatin immunoprecipitation to identify direct SRY target genes. Anti-mouse SRY antibody precipitated a region 7.5 kb upstream of the transcriptional start site of cerebellin 4 precursor (Cbln4), which encodes a secreted protein. Cbln4 is expressed in Sertoli cells in the developing gonad, with a profile mimicking that of the testis-determining gene SRY-box containing gene 9 (Sox9). In transgenic XY mouse embryos with reduced Sox9 expression, Cbln4 expression also was reduced, whereas overexpression of Sox9 in XX mice caused an upregulation of Cbln4 expression. Finally, ectopic upregulation of SRY in vivo resulted in ectopic expression of Cbln4. Our findings suggest that both SRY and SOX9 contribute to the male-specific upregulation of Cbln4 in the developing testis, and they identified a direct in vivo target gene of SRY.</div>
</front>
</TEI>
<affiliations><list><country><li>Australie</li>
<li>France</li>
<li>Japon</li>
</country>
<region><li>Provence-Alpes-Côte d'Azur</li>
<li>Région de Kantō</li>
<li>Victoria (État)</li>
</region>
<settlement><li>Melbourne</li>
<li>Nice</li>
<li>Tokyo</li>
</settlement>
<orgName><li>Université de Melbourne</li>
<li>Université de Tokyo</li>
</orgName>
</list>
<tree><country name="Australie"><noRegion><name sortKey="Bradford, Stephen T" sort="Bradford, Stephen T" uniqKey="Bradford S" first="Stephen T." last="Bradford">Stephen T. Bradford</name>
</noRegion>
<name sortKey="Bernard, Pascal" sort="Bernard, Pascal" uniqKey="Bernard P" first="Pascal" last="Bernard">Pascal Bernard</name>
<name sortKey="Harley, Vincent" sort="Harley, Vincent" uniqKey="Harley V" first="Vincent" last="Harley">Vincent Harley</name>
<name sortKey="Koopman, Peter" sort="Koopman, Peter" uniqKey="Koopman P" first="Peter" last="Koopman">Peter Koopman</name>
<name sortKey="Maddugoda, Madhavi P" sort="Maddugoda, Madhavi P" uniqKey="Maddugoda M" first="Madhavi P." last="Maddugoda">Madhavi P. Maddugoda</name>
<name sortKey="Sinclair, Andrew" sort="Sinclair, Andrew" uniqKey="Sinclair A" first="Andrew" last="Sinclair">Andrew Sinclair</name>
<name sortKey="Wilhelm, Dagmar" sort="Wilhelm, Dagmar" uniqKey="Wilhelm D" first="Dagmar" last="Wilhelm">Dagmar Wilhelm</name>
</country>
<country name="Japon"><region name="Région de Kantō"><name sortKey="Hiramatsu, Ryuji" sort="Hiramatsu, Ryuji" uniqKey="Hiramatsu R" first="Ryuji" last="Hiramatsu">Ryuji Hiramatsu</name>
</region>
<name sortKey="Kanai, Yoshiakira" sort="Kanai, Yoshiakira" uniqKey="Kanai Y" first="Yoshiakira" last="Kanai">Yoshiakira Kanai</name>
</country>
<country name="France"><region name="Provence-Alpes-Côte d'Azur"><name sortKey="Chaboissier, Marie Christine" sort="Chaboissier, Marie Christine" uniqKey="Chaboissier M" first="Marie-Christine" last="Chaboissier">Marie-Christine Chaboissier</name>
</region>
<name sortKey="Chaboissier, Marie Christine" sort="Chaboissier, Marie Christine" uniqKey="Chaboissier M" first="Marie-Christine" last="Chaboissier">Marie-Christine Chaboissier</name>
<name sortKey="Schedl, Andreas" sort="Schedl, Andreas" uniqKey="Schedl A" first="Andreas" last="Schedl">Andreas Schedl</name>
<name sortKey="Schedl, Andreas" sort="Schedl, Andreas" uniqKey="Schedl A" first="Andreas" last="Schedl">Andreas Schedl</name>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Wicri/Asie/explor/AustralieFrV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 008043 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 008043 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Wicri/Asie |area= AustralieFrV1 |flux= Main |étape= Exploration |type= RBID |clé= Pascal:10-0036251 |texte= The Cerebellin 4 Precursor Gene Is a Direct Target of SRY and SOX9 in Mice }}
This area was generated with Dilib version V0.6.33. |